The three-dimensional structures of S-layers of two novel Eubacterium species isolated from inflammatory human processes |
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Authors: | A. Sjö gren,D. N. Wang,S. Hovmö ller,M. Haapasalo,H. Ranta,E. Kerosuo,H. Jousimies-Somer,K. Lounatmaa |
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Affiliation: | Department of Structural Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden. |
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Abstract: | The three-dimensional structures of the crystalline surface layers of two species of Eubacteria have been determined by electron microscopy and computerized image processing. The S-layer of Eubacterium sp. ES4C has tetragonal symmetry, with a unit cell spacing of 10.6 nm and a thickness of 9.5 nm, while that of Eubacterium sp. AHN 990 has hexagonal symmetry a = b = 15.7 nm and a thickness of 13 nm. The resolutions in the reconstructions were 2.5 nm and 1.8 nm, respectively. The reconstruction of the S-layer of strain ES4C reveals a distinct domain structure: a major tetramer, arms connecting adjacent unit cells, and a minor tetramer. The S-layer of strain AHN 990, on the other hand, has a rather complex arrangement, centred around the six-fold axis. |
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