| 产喜树碱内生真菌的筛选及鉴定 |
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| 引用本文： | 王玉美,陈洪章.产喜树碱内生真菌的筛选及鉴定[J].微生物学通报,2011,38(6):884-888. |
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| 作者姓名： | 王玉美 陈洪章 |
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| 作者单位： | 中国科学院过程工程研究所生化工程国家重点实验室,北京,100190 |
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| 基金项目： | 国家973计划项目(No. 2004CB719700); 中国科学院知识创新工程重要方向性项目(No. KJCX2-SW-206); “十一五”国家科技支撑计划项目(No. 2007BAD39B01) |
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| 摘 要： | 从喜树Camptotheca acuminate树皮和果实中分离得到27株内生真菌,发酵后经HPLC检测,筛选出一株菌丝产喜树碱的菌,产量达774μg/L。对其ITS序列进行系统发育分析,结合其培养特征和显微特征,鉴定为拟茎点霉属(Phomopsis sp.)。这是首次报道分离自喜树的该属真菌发酵产喜树碱。
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| 关 键 词： | 喜树 内生真菌 喜树碱 拟茎点霉属 系统发育分析 |
Isolation and identification of a camptothecin-producing endophytic fungus from Camptotheca acuminate |
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| WANG Yu-Mei and CHEN Hong-Zhang.Isolation and identification of a camptothecin-producing endophytic fungus from Camptotheca acuminate[J].Microbiology,2011,38(6):884-888. |
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| Authors: | WANG Yu-Mei and CHEN Hong-Zhang |
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| Institution: | National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China |
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| Abstract: | 27 endophytic fungi were isolated from the barks and fruits of Camptotheca acuminate and then cultured in submerged fermentation.The fermentation products of each strain were analyzed with high performance liquid chromatography(HPLC) which showed that one produced camptothecin with the output of 774 μg/L.The strain was identified as Phomopsis sp.by phylogenetic analysis based on ITS rDNA and cultural and morphological characteristics.This was the first report that Phomopsis sp.,which was isolated from Camptotheca acuminate,produced camptothecin. |
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| Keywords: | Camptotheca acuminate Endophytic fungi Camptothecin Phomopsis sp Phylogenetic analysis |
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