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| 一株抗MRSA内生细菌的鉴定及其活性物质 | |
| 引用本文： | 张守村,韩松,黄晓艳,梅文莉,林天兴,龚明福.一株抗MRSA内生细菌的鉴定及其活性物质[J].微生物学通报,2011,38(6):860-864. |
| 作者姓名： | 张守村 韩松 黄晓艳 梅文莉 林天兴 龚明福 |
| 作者单位： | 1. 塔里木大学生命科学学院 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室，新疆阿拉尔843300;农业部热带农业生物技术重点实验室 中国热带农业科学院热带农业生物技术研究所，海南海口571101 2. 塔里木大学生命科学学院 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔,843300 3. 农业部热带农业生物技术重点实验室 中国热带农业科学院热带农业生物技术研究所,海南海口,571101 4. 乐山师范学院化学与生命学院,四川乐山,634004 |
| 基金项目： | 国家自然科学基金项目(No. 30960010); 塔里木大学校长基金重点项目(No. TDZKZD06001) |
| 摘 要： | 根据生理生化特征和16S rDNA序列,对一株从新疆苦豆子Sophora alopecuroides中分离的、对耐甲氧西林金黄色葡萄球菌(Methicillin-resistant Staphylococcus aureus,MRSA)具有较强拮抗作用的内生细菌菌株KDNB6进行鉴定;并通过硅胶柱、凝胶柱层析从该菌发酵产物中分离纯化了抗MRSA的活性物质。结果表明菌株KDNB6各项特征与粘质沙雷氏菌Serratia marcescens最为接近,鉴定为S.marcescens。从菌株KDNB6的代谢产物中分离到一个抗MRSA的活性成分。 |
| 关 键 词： | MRSA 内生细菌 粘质沙雷氏菌 苦豆子 鉴定 |
Identification of endophytic bacteria KDNB6 from Sophora alopecuroides and antibacterial substance against MRSA |
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| ZHANG Shou-Cun,HAN Song,HUANG Xiao-Yan,MEI Wen-Li,LIN Tian-Xing and GONG Ming-Fu.Identification of endophytic bacteria KDNB6 from Sophora alopecuroides and antibacterial substance against MRSA[J].Microbiology,2011,38(6):860-864. | |
| Authors: | ZHANG Shou-Cun HAN Song HUANG Xiao-Yan MEI Wen-Li LIN Tian-Xing and GONG Ming-Fu |
| Institution: | 1. Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang Production Construction Corps, College of Life Science, Tarim University, Alar, Xinjiang 843300, China; 2. Key Laboratory of Tropical Crop Bio-technology, Min;1. Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang Production Construction Corps, College of Life Science, Tarim University, Alar, Xinjiang 843300, China;1. Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang Production Construction Corps, College of Life Science, Tarim University, Alar, Xinjiang 843300, China;2. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China;3. School of Chemistry and Life Sciences, Leshan Normal University, Leshan, Sichuan, 634004, China;1. Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang Production Construction Corps, College of Life Science, Tarim University, Alar, Xinjiang 843300, China |
| Abstract: | An endophytic bacterium isolated from Sophora alopecuroides in Xinjiang, which had a conspicuous antagonistic effect on methicillin-resistant Staphylococcus aureus (MRSA), was studied and bioactive substances were separated and purified. Strain KDNB6 was identified as Serratia marcescens by its morphological, physiological and biochemical properties and 16S rDNA sequences. An anti-MRSA active component produced in fermentation broth was separated and purified by silica gel column chromatography and sephadex gel column chromatography. |
| Keywords: | MRSA Endophytic bacteria Serratia marcescens Sophora alopecuroides Identification |
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