Steroidogenic effect of exogenous phospholipase C on bovine adrenal fasciculata cells |
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Authors: | A J Hadjian M Culty E M Chambaz |
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Institution: | Laboratoire de Biochimie Endocrinienne, INSERM U-244, CNRS ERA-942, Université Scientifique et Médicale de Grenoble, 38402 Saint Martin d''Hères Cédex, France |
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Abstract: | Phospholipase C (Bacillus cereus) added to the incubation medium stimulated the steroidogenic activity of bovine adrenal zona fasciculata cell suspensions to a level similar to that induced by optimal concentration of ACTH. This effect was not related to an increase of cyclic AMP; it was calcium-dependent and was also induced by an other bacterial phospholipase C (from Clostridium perfringens) whereas phospholipases A2 and D were ineffective. Phospholipid metabolism was examined in these cells after radiolabeling with 14C]-glycerol or 32P]orthophosphate. Phospholipase C induced a very fast (5 seconds) increase in cellular 14C]-1,2-diacylglycerol followed by 32P] labeling of phosphatidic acid and phosphatidylinositol. These events preceded the stimulation of steroidogenesis which was detectable after 2 minutes of incubation. These observations suggest that activation of an endogenous phospholipase C activity may be considered as an early event in the response of bovine adrenocortical cells to steroidogenic effectors such as angiotensin II and acetylcholine. |
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