Isolation of a urinary digitalis-like factor indistinguishable from digoxin |
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| Authors: | A Goto T Ishiguro K Yamada M Ishii M Yoshioka C Eguchi M Shimora T Sugimoto |
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| Institution: | Second Department of Internal Medicine, University of Tokyo, Japan. |
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| Abstract: | A digitalis-like factor has been purified to apparent homogeneity from human urine based on the inhibitory effect on 3H] ouabain binding to intact human erythrocytes. The purification scheme involved large scale adsorption followed by preparative, semipreparative and analytical high-performance liquid chromatography. The purified material showed a prominent digoxin-like immunoreactivity. The behaviour of the isolated substance was identical to that of authentic digoxin in three high-performance liquid chromatography and three thin-layer chromatography systems. Moreover, fast atom bombardment mass spectrum and proton nuclear magnetic resonance spectrum suggested that the purified material may be indistinguishable from digoxin. |
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