Preparation of delipidized serum protein for use in cell culture systems

Summary A rapid procedure for the preparation of delipidized serum protein is described. The delipidized protein can be used for the maintenance and growth of tissue culture cells in a lipid-free environment. The extraction procedure greatly reduces all serum lipid classes and the delipidized protein supports the growth of a variety of cells in culture. This research was supported in part by USPHS research grants HL-09103 and HL-16058 from the National Heart and Lung Institute and RR-05540 from the Division of Research Resources. This work was done during tenure as Established Investigator of the American Heart Association (G.H.R.).