The secretory ameloblast of the mini-pig foetus |
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Authors: | M. E. Matthiessen M.D. Ph.D. P. Rømert |
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Affiliation: | (1) Anatomy Departments A and C, University of Copenhagen, Universitetsparken 1, DK-2100 Copenhagen Ø, Denmark;(2) Anatomy Department A, Universitetsparken 1, DK-2100 Copenhagen Ø, Denmark |
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Abstract: | Electron microscopic investigations of secretory ameloblasts from deciduous tooth germs of mini-pig foetuses and investigations of the ability of various fixatives to preserve these cells in tooth germs immersion-fixed in toto 5 min, 10 min, 15 min, 20 min and 40 min after death of the mother gave the following results:1. | The ameloblasts exhibit ultrastructural characteristics typical of exocrine secretory cells of merocrine type. | 2. | The localization of organelles is as in rodent secretory ameloblasts, but differs from the location in the human analogues. | 3. | Fixation with 4% formaldehyde invariably gives unacceptable ameloblast preservation. | 4. | Fixation with 2.5% glutaraldehyde gives fair preservation of the ameloblasts when the germs are fixed within 10 min of the death of the mother. | 5. | Fixation with a fixative mixture 2% formaldehyde — 1.25% glutaraldehyde gives good preservation when the ameloblasts are fixed within 15 min of the death of the mother. | 6. | Fixation with a fixative mixture 2% formaldehyde — 1% glutaraldehyde — 1% acrolein gives good ameloblast preservation when the germs are fixed within 15 min of the death of the mother. |
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Keywords: | Ameloblast Mini-pig Foetus Tooth Fixation |
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