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Alternative carbohydrates promote differentiation of plant cells
Authors:A M Kinnersley  W E Henderson
Institution:(1) Moffett Technical Center, CPC International, Box 345, 60501 Summit-Argo, IL, USA;(2) Present address: Phyton Technologies, Inc., Science and Technology Center, 1299 Bethel Valley Road, 37830 Oak Ridge, TN, USA;(3) Present address: Enzyme Biosystems, 3350 Salt Creek Lane, Suite 103, 60005 Arlington Heights, IL, USA
Abstract:Corn syrups have been evaluated in media for embryogenesis, androgenesis and the production of secondary metabolites from plant tissue culture. In the systems examined, higher productivity was obtained with media containing corn syrups than with comparable media containing glucose or sucrose. Corn syrup did not increase growth of unorganized cell cultures. Increased productivity therefore reflects a syrup-mediated promotion of cell differentiation. The effects of corn syrup on increasing yields of secondary metabolites were evident only after several passages in syrup-containing medium. This shows the importance of monitoring production over several passages to determine the effect of different carbon sources on secondary metabolite production. Superiority of the syrup is due primarily to the component sugars maltose and glucose. Mixtures of these sugars gave higher yields of secondary products than either sugar used alone.Abbreviations DP degree of polymerization
Keywords:carbohydrate  maltose  corn syrup  carbon source  plant tissue culture media  secondary products  embryogenesis
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