Synthesis of glycinin in a wheat germ cell-free system.

Polyribosomes isolated from the cotyledons of developing soybean seeds were translated in a wheat germ cell-free system. When the radioactive translation products synthesized in the cell-free system were fractionated by centrifugation on sucrose density gradient, a radioactive peak which overlapped an authentic glycinin was detected. This radioactive co-sedimentable material was judged to be also a glycinin by its behavior toward polyacrylamide gel electrophoresis and immunoprecipitation.