Discrimination of species and strains of basidiomycete genusCoprinus by random amplified polymorphic DNA (RAPD) analysis |
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Authors: | Yasuhiro Ito Tsutomu Fushimi Sonoe O Yanagi |
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Institution: | (1) National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, 2-1-2, Kannondai, 305-8642 Tsukuba, Japan |
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Abstract: | All five examined strains ofCoprinus cinereus could be clearly discriminated from the strains of five otherCoprinus species by RAPD patterns with 12 of 13 primers. Also one specimen of unknownCoprinus strain was identified to beC. cinereus by this method. The RAPD patterns were similar among the strains in the same species; many common DNA fragments were recognized
as well as some strain-specific DNA fragments. Thus all seven strains ofC. cinereus and all four strains ofC. angulatus examined could be distinguished individually. Diakryotic strains showed the combined RAPD patterns of the two monokaryotic
strains constituting the dikaryon. The combined RAPD markers observed in the dikaryons were segregated in their basidiospore
progeny. All 18 randomly picked progeny showed different combinations of RAPD markers from the parental strains. |
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Keywords: | Coprinus RAPD strain identification |
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