Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori |
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| Authors: | I Faus C del Moral N Adroer J L del Río C Patiño H Sisniega C Casas J Bladé V Rubio |
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| Institution: | (1) URQUIMA, S.A. (URIACH Group), Arnau de Vilanova 22–42, E-08015 Sant Fost de Campsentelles (Barcelona), Spain Tel.: +34 3 570 5450 Fax: +34 3 570 2476, ES;(2) Centro Nacional de Biotecnología (CSIC-UAM), Campus Canto Blanco, E-28049 Madrid, Spain, ES;(3) Unitat d'Enginyería Química, Facultat de Ciencies, Universitat Autónoma de Barcelona, 08193 Bellaterra (Barcelona), Spain, ES |
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| Abstract: | A recombinant form of the sweet-tasting protein thaumatin has been produced in the filamentous fungus Aspergillus niger var. awamori. Expression cassettes containing a synthetic gene encoding thaumatin II were prepared and used to transform Aspergillus niger var. awamori strain NRRL312. Several fungal strains capable of synthesizing and secreting thaumatin into the culture medium were generated,
and their production capabilities were determined, first in shake flasks and later in a laboratory fermentor. We report the
expression and secretion of thaumatin in concentrations of 5–7 mg/l. This recombinant thaumatin is sweet.
Received: 7 October 1997 / Received revision: 21 November 1997 / Accepted: 21 November 1997 |
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