Polyethylene Glycol-Modified Lipase Soluble and Active in Organic Solvents |
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| Authors: | Yuji Inada Ayako Matsushima Katsunobu Takahashi Yuji Saito |
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| Institution: |
a Department of Materials Science and Technology, Toin University of Yokohama, Midori-ku, Yokohama, Japan
b Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, Meguro-ku Tokyo |
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| Abstract: | A new approach in biotechnological processes is to use lipase modified with polyethylene glycol(PEG) which has both hydrophilic and hydrophobic properties. The PEG-lipase is soluble in organic solvents such as benzene and chlorinated hydrocarbons and exhibits high enzymic activity in organic solvents. The PEG-lipase catalyses the reverse reaction of hydrolysis in organic solvents; ester synthesis and ester exchange reactions. The PEG-lipase can also be conjugated to magnetite (Fe3O4). The magnetic lipase catalyses ester synthesis in organic solvents and can be readily recovered by magnetic force without loss of enzymic activity. |
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| Keywords: | Lipase Polyethylene glycol ester synthesis ester exchange magnetite |
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