Extracellular Interaction between hCD98 and the PDZ Class II Domain of hCASK in Intestinal Epithelia |
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Authors: | Yutao Yan Sona Vasudevan Hang Nguyen Ulrich Bork Shanthi Sitaraman Didier Merlin |
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Institution: | Division of Digestive Diseases, Department of Medicine, Emory University School of Medicine, 615 Michael Street, Atlanta, GA 30322, USA. |
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Abstract: | The extracellular domain of the glycoprotein-associated integrin hCD98 protrudes into the basolateral extracellular space of the intestine and contains a PDZ class II-binding domain (GLLLRFPYAA, amino acids 520-529). Protein-protein interaction studies in vitro as well as in human colonic sections and Caco2-BBE cells have revealed that hCD98 coimmunoprecipitated with the basolateral membrane-associated guanylate kinase hCASK and that this interaction occurred in a PDZ domain-dependent manner. These novel results, which provide the first evidence for a PDZ domain-dependent interaction between a membrane protein and an extracellular protein, open a new field of investigation related to extracellular signaling in cell biology. |
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Keywords: | Extracellular C terminus hCD98 hCASK PDZ class II Caco2-BBE |
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