Clonal propagation of Acacia catechu Willd. by shoot tip culture |
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Authors: | Kulneet Kaur U Kant |
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Institution: | (1) Plant Pathology and Tissue Culture Biotechnology Lab., Department of Botany, University of Rajasthan, Jaipur, 302 004, India;(2) Plant Pathology and Tissue Culture Biotechnology Lab., Department of Botany, University of Rajasthan, Jaipur, 302 004, India |
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Abstract: | A method is described for in vitromicropropagation through shoot apices of Acaciacatechu Willd., a semi-arid tree valued for Katha (atanin-like substance obtained from red heart wood of10–20 year old trees) and timber. Explants wereexcised from 15-days-old in vitro grownseedlings raised from superior seed stocks. Shoot budinduction from shoot apex explants was observed onMurashige and Skoog's (MS) 12] medium containingvarious growth regulators. A maximum of 12 shoots wasobtained on MS medium supplemented with 1.5 mg/l 6-benzylaminopurine (BAP) and 1.5 mg/l kinetin.Well-developed shoots (3–4 cm long) were rooted on
strength MS medium with 3.0 mg/l indole-3-acetic acid (IAA) and sucrose 1.5%. In vitro regenerated plantlets of A. catechu were transferred to field conditions. |
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Keywords: | Acacia catechu plantlets shoot tip tissue culture |
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