Genetic analysis of inbreeding of two strains of the parasitic nematode Haemonchus contortus |
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Authors: | Roos Marleen H Otsen Myrthe Hoekstra Ruurdtje Veenstra Jetty G Lenstra Johannes A |
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Institution: | Department of Molecular Recognition, Institute for Animal Science and Health (ID-Lelystad), Lelystad, The Netherlands. m.h.roos@planet.nl |
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Abstract: | Haemonchus contortus is a sheep parasitic nematode that causes severe economic losses. Previous studies have indicated a high degree of genetic heterogeneity, which is hardly affected by selection for drug resistance. As a tool for the analysis of the population dynamics of H. contortus and its response to drug resistance, we designed a strategy to study the inbreeding of a benzimidazole-sensitive and a benzimidazole-resistant strain. After 15 generations, a theoretical inbreeding coefficient of 0.87 was achieved. The different stages of inbreeding were analysed using restriction fragment polymorphism, microsatellite variability and amplified fragment length polymorphism. Model-based clustering of the amplified fragment length polymorphism genotypes showed that the allele frequencies of the benzimidazole-resistant strain were stable during the last eight generations. In the sensitive strain a gradual shift of allele frequencies was observed, which led to a temporary increase of the genetic diversity around the eight generations. |
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Keywords: | Haemonchus contortus Nematode Anthelmintics Drug resistance Amplified fragment length polymorphism Inbreeding |
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