三羟异黄酮对HER-2／neu高表达乳腺癌细胞血管生成相关因子表达的影响

三羟异黄酮(genistein)是大豆中的一种非营养成分，其结构与黄酮化合物类似，能竞争性地与雌激素受体结合，故称之为植物雌激素(phytoestmgen)。它具有广泛的生物学作用，如抗肿瘤、抗病毒、抗真菌、抗氧化、抗突变、抗高血压、抗增生等，其中genistein抑制肿瘤的血管生成是当前研究的热点之一。

Effect of genistein on expression of angiogenesis related factors in HER-2/neu-overexpressing breast cancer cells]

To study the molecular mechanism of inhibition of angiogenesis in HER-2/neu-overexpressing breast cancer by genistein, HER-2/neu negative expression breast cancer MCF-7 cells were transfected with HER-2/neu to establish HER-2/neu-overexpressing MCF-7 cells (named MCF-7/HER-2). Expression of vascular endothelial growth factor (VEGF), urokinase-type plasminogen activator (uPA), matrix metalloproteinase-2,9 (MMP-2,9) in MCF-7, MCF-7/HER-2 as well as genistein-treated MCF-7/HER-2 were measured by immunocytochemistry and Western blot. We found that the expression of VEGF, MMP-2,9 and uPA in MCF-7/HER-2 cells were highter than that in MCF-7 cells, those angiogenesis related factors expression in MCF-7/HER-2 cells significantly decreased after treatment with genistein. Genistein could inhibit expression of angiogenesis-related factors VEGF, MMP-2,9 and uPA in HER-2/neu-overexpressing breast cancer cells, and this may be part of molecular mechanism of its anti-angiogenesis in HER-2/neu-overexpressing breast cancer.