Thermally modified azocasein--a new insoluble substrate for the determination of proteolytic activity |
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| Authors: | I Safarík |
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| Institution: | Laboratory of Analytical Chemistry, Czechoslovak Academy of Sciences, Ceské Bud?jovice. |
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| Abstract: | An insoluble chromogenic substrate for the determination of proteolytic activity was prepared by heating azocasein in a thin layer at 200 degrees C for 4 h in a hot-air thermostat. The activity of an extracellular bacterial proteinase produced by Bacillus sp. was determined with this new substrate. |
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