Recovery of RNA from flow-sorted fixed cells. |
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Authors: | S Khochbin D Grunwald M Pabion J J Lawrence |
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Affiliation: | Laboratoire de Biologie Moléculaire du Cycle Cellulaire, Unité INSERM 309, Grenoble, France. |
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Abstract: | We describe a method for the preparation of RNA from ethanol-fixed cells, allowing analysis of the RNA from cells "frozen" in a given physiological state. This technique may have important applications in experiments which require prolonged cell manipulations before RNA preparation, such as investigations of cell-cycle-regulated gene expression, which require the preparation of cells for cell-cycle flow analysis, and even for long-term cell sorting. It eliminates all the inconveniences associated with the use of fresh cells, and allows cell-cycle biologists to couple flow cytometry methodology with the advancing techniques of molecular biology. |
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