| 人干细胞转录因子Nanog和BTB/POZ家族蛋白NAC1的相互作用 |
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| 引用本文： | 韩正滨,林艳丽,熊福银,田利源,周艳荣,邓继先,陈红星.人干细胞转录因子Nanog和BTB/POZ家族蛋白NAC1的相互作用[J].生物技术通讯,2008,19(4):503-505. |
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| 作者姓名： | 韩正滨 林艳丽 熊福银 田利源 周艳荣 邓继先 陈红星 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100071 |
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| 摘 要： | 目的：研究人干细胞转录因子Nanog和BTB/POZ家族蛋白NAC1的相互作用,并初步确定作用区域。方法：应用免疫共沉淀、GSTpull-down实验验证人Nanog与NAC1的相互作用。结果：人Nanog与NAC1能够相互作用,且NAC1的BTB/POZ结构域对于二者相互作用是必需的。结论：人Nanog和NAC1在体内、外均能形成复合物,二者的相互作用对于人胚胎干细胞的自我更新及肿瘤的发生可能具有重要的作用。
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| 关 键 词： | Nanog NAC1 GST pull-down 免疫共沉淀 |
Human Nanog Interacts with the BTB/POZ Protein NAC1 |
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| HAN Zheng-Bin,LIN Yan-Li,XIONG Fu-Yin,TIAN Li-Yuan,ZHOU Yan-Rong,DENG Ji-Xian,CHEN Hong-Xing.Human Nanog Interacts with the BTB/POZ Protein NAC1[J].Letters in Biotechnology,2008,19(4):503-505. |
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| Authors: | HAN Zheng-Bin LIN Yan-Li XIONG Fu-Yin TIAN Li-Yuan ZHOU Yan-Rong DENG Ji-Xian CHEN Hong-Xing |
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| Institution: | (Beijing Institute of Biotechnology,Beijing 100071,China ) |
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| Abstract: | Objective：To investigate the interaction between human Nanog and the BTB/POZ protein NAC1,and to define the regions that are essential for the binding between Nanog and NAC1.Methods：The interaction was demonstrated by co-immunoprecipition and GST pull-down.Results：The interaction between Nanog and NAC1 was validated and the BTB/POZ domain of NAC1 was indispensable to the interaction.Conclusion：Nanog could bind NAC1 in vivo and in vitro.The interaction between these two proteins may contribute to the self-renewal of human embryonic stem cells as to the tumorgenesis procedure. |
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| Keywords: | Nanog NAC1 GST pull-down |
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