cis-Diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific |
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| Authors: | Z M Banjar L S Hnilica R C Briggs E Dominguez J L Stein G S Stein |
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| Institution: | 1. Departments of Biochemistry and Pathology, A. B. Hancock Jr., Memorial Laboratory of the Vanderbilt University Cancer Center, and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, U.S.A.;1. Department of Biochemistry and Molecular Biology, University of Florida School of Medicine, Gainesville, Florida 32610 U.S.A.;1. MOE Key Laboratory of Wooden Material Science and Application, Beijing Forestry University, Beijing 100083, China;2. Beijing Key Laboratory of Wood Science and Engineering, Beijing Forestry University, Beijing 100083, China;1. Department of Chemical and Biological Engineering, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea;2. Department of Chemical and Biomolecular Engineering, Sogang University, Sinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea |
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| Abstract: | Immunochemical analysis was employed to investigate the cell cycle-dependent protein-DNA crosslinking by cis-diamminedichloroplatinum II (cis-DDP), in HeLa-S3 cells. Cells synchronized by double thymidine block or hydroxyurea were released into S phase and incubated at 2-h intervals with cis-DDP as they progressed through S1, G2, M, and then into G1 and S phases of the subsequent cycle. Immunoblots of the DNA-crosslinked antigens reacted with antisera to 0.35 M NaCl extract or residue of HeLa S-phase nuclei revealed that several antigens changed their DNA-crosslinking pattern during the progression of HeLa cells through their reproductive cycle. |
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