| 不同因素对岩黄连悬浮细胞生长的影响 |
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| 引用本文： | 程华,余龙江.不同因素对岩黄连悬浮细胞生长的影响[J].生物技术,2007,17(3):56-59. |
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| 作者姓名： | 程华 余龙江 |
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| 作者单位： | 1. 武汉科技大学医学院,湖北,武汉,430065;武汉烟草(集团)有限公司技术中心,湖北,武汉,430051
2. 华中科技大学生命科学与技术学院,湖北,武汉,430074 |
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| 摘 要： | 目的:建立一个快速生长的岩黄连悬浮细胞培养体系。方法:研究了接种量、基本培养基、初始pH值、不同碳源对岩黄连悬浮细胞生长的影响。结果:合适的接种量是7.5~10%(FW),接种量过少会抑制细胞生长;B5和MS基本培养基均适合岩黄连细胞的生长;最佳初始培养基pH值为6.0,此时获得的细胞生物量最高;岩黄连悬浮细胞培养的生长周期为24d,最大生物量出现在第18d,达到14.1g/l(DW);蔗糖比葡萄糖更有利于岩黄连细胞的生长,添加60g/l蔗糖所获得的生物量最高,达到18.5g/l(DW)。
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| 关 键 词： | 悬浮细胞培养 岩黄连 接种量 |
| 文章编号： | 1004-311X（2007）03-0056-04 |
| 收稿时间： | 2006-12-21 |
| 修稿时间： | 2006-12-212007-03-09 |
Effects of Different Factors on Cell Growth of Corydalis saxicola Bunting Suspension Cultures |
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| CHENG Hua,YU Long-jiang.Effects of Different Factors on Cell Growth of Corydalis saxicola Bunting Suspension Cultures[J].Biotechnology,2007,17(3):56-59. |
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| Authors: | CHENG Hua YU Long-jiang |
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| Institution: | 1. Medical College, Wuhan University of Science and Technology, Wuhan 430065, China; 2. Center of Technology, Wuhan Tobacco Co. Lid, Wuhan 430051, China;3. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China |
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| Abstract: | Objective:Cell suspension cultures were established through selection of inoculum size.Methods:Basal medium, initial pH value, and carbon resource. Results:An inoculum size of 7.5-10% (FW) was suitable for cell growth and small inoculum size could impress biomass accumulation. The B5 and MS basal medium were fit to cell growth, and the favorable initial pH value of medium was 6.0. The growth period of C. saxicola cell cultures was around 24d with highest biomass on day 18. Sucrose was much better than glucose for cell growth; the addition of 60g/l sucrose produced the most biomass with 18.5g/l (DW). |
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| Keywords: | cell suspension culture Corydalis saxicola Bunting inoculum size |
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