Uptake of L-leucine by Trout Red Blood Cells and Peripheral Lymphocytes |
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Authors: | MA Gallardo JL Albi J Sánchez |
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Institution: | (1) Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 645, E-08028 Barcelona, Spain, ES |
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Abstract: | The uptake of l-leucine by trout red blood cells and peripheral lymphocytes has been analyzed. The present study shows two functionally different
Na+-independent systems for apolar branched-chain amino acids. They are designated as L systems because they share some properties
with the mammalian L system. The carrier present in red blood cells has low K
m
values, is trans-stimulable and not stereospecific for leucine uptake; on the other hand, the system present in lymphocytes is stereospecific
for leucine uptake and trans-inhibitable. Both carriers are pH sensitive in a similar fashion at low pHs, but there are important differences at higher
pH values (above neutrality). These properties are compared with these of the asc systems previously reported in these cells.
Received: 2 June 1995/Revised: 7 March 1996 |
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Keywords: | : Leucine uptake — Red blood cell (RBC) — Peripheral blood lymphocytes (PBL) — L system — Trout (Salmo trutta) |
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