Optimised affinity purification of polyclonal antibodies from hyper immunised ovine serum using a synthetic Protein A adsorbent, MAbsorbent A2P |
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Authors: | Newcombe Anthony R Cresswell Chrissie Davies Susannah Watson Keith Harris Guy O'Donovan Kieran Francis Richard |
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Institution: | Process Development Group, Protherics Ltd., Blaenwaun, Ffostrasol, Llandysul, Wales SA44 5JT, UK. tony.newcombe@protherics.com |
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Abstract: | This report describes the applicability of a synthetic chromatography adsorbent for large-scale purification of polyclonal immunoglobulin G from hyper immunised ovine serum. Under optimised conditions, MAbsorbent A2P was shown to bind approximately 27 mg mL(-1) of ovine immunoglobulin from undiluted serum, with eluted IgG purities of >95%, minor levels of albumin (approximately 1%) and undetectable levels of leached ligand in the purified preparations. The results presented here indicate that the optimised affinity capture of immunoglobulin from ovine serum using MAbsorbent A2P is a feasible alternative to Protein A chromatography or sodium sulphate precipitation for the initial capture of antibodies from undiluted serum. |
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