| 简易的沙眼衣原体扩增及纯化方法的建立 |
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| 引用本文： | 王玲,单路鹃,邱阳,郭莉,刘越坚,吴泰华.简易的沙眼衣原体扩增及纯化方法的建立[J].中国微生态学杂志,2004,16(6):374-375. |
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| 作者姓名： | 王玲 单路鹃 邱阳 郭莉 刘越坚 吴泰华 |
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| 作者单位： | 大连医科大学附属第一医院,中心实验室,辽宁,大连,116011 |
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| 摘 要： | 目的建立扩增培养和纯化沙眼衣原体的简化方法.方法采用HeLa细胞培养扩增衣原体,并应用一步法将沙眼衣原体纯化回收.结果获得了具有高感染活性的沙眼衣原体.结论该方法与常规方法相比更简便、快速,同时减少污染机会、不需要超声和超速离心机等设备.
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| 关 键 词： | 沙眼衣原体 细胞培养 纯化 |
| 文章编号： | 1005-376X(2004)06-0374-02 |
| 修稿时间： | 2004年5月21日 |
Simplified methods for propagation and purification of Chlamydia trachomatis |
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| WANG Ling,SHAN Lu-juan,QIU Yang,et al..Simplified methods for propagation and purification of Chlamydia trachomatis[J].Chinese Journal of Microecology,2004,16(6):374-375. |
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| Authors: | WANG Ling SHAN Lu-juan QIU Yang |
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| Abstract: | Objective To establish simplified methods of Chlamydia trachomatis propagation and purification.Methods Chlamydia trachomatis was proliferated in HeLa cell monolayers with IMDM culture medium and purified by one-step method.Results A high titer Chlamydia trachomatis stock was obstained.Conclusion The method was simpler and faster it could reduce the contaminating chance and machine requirement compared with routine methods. |
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| Keywords: | Chlamydia trachomatis Cell culture Purification |
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