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Isolation and characterization of eight polymorphic microsatellite loci for the Mediterranean gorgonian Paramuricea clavata
Authors:Gemma Agell  Marc Rius  Marta Pascual
Institution:1. Centre d’Estudis Avan?ats de Blanes (CSIC), C/Accés a la Cala St. Francesc, 14, 17300, Blanes, Spain
2. Departament de Biologia Animal, Universitat de Barcelona, Av. Diagonal 645, 08028, Barcelona, Spain
3. Departament de Genètica, Universitat de Barcelona, Av. Diagonal 645, 08028, Barcelona, Spain
Abstract:The gorgonian Paramuricea clavata is a benthic organism often included in conservation management plans since it creates complexity in the ecosystems and is extremely vulnerable to disturbances. Eight microsatellite markers isolated from an enriched genomic library were characterized in a total of 50 individuals from two north-western Mediterranean populations. All loci were polymorphic and the number of alleles per locus ranged between 2 and 14. Significant genetic differentiation was observed between populations. The polymorphic markers presently isolated will allow for the assessment of the spatial genetic structure between and within populations of this umbrella species of the Mediterranean Sea.
Keywords:
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