| SLP-2荧光表达载体的构建及其在乳腺癌细胞MCF-7中的表达与定位 |
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| 引用本文： | 王义军,吴文韬,徐甜,向双林,张健,韩梅.SLP-2荧光表达载体的构建及其在乳腺癌细胞MCF-7中的表达与定位[J].激光生物学报,2010,19(1):10-13,24. |
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| 作者姓名： | 王义军 吴文韬 徐甜 向双林 张健 韩梅 |
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| 作者单位： | 湖南师范大学生命科学学院,湖南长沙,410081 |
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| 基金项目： | 国家973资助项目，长沙市科技厅计划项目 |
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| 摘 要： | 人的SLP-2基因是一个新的肿瘤相关基因，它在多个癌组织里高表达，如食管鳞状细胞癌组织和肺癌组织。其中在乳腺癌组织的高表达和病人的存活率负相关，这说明该基因很可能在乳腺癌的发生中发挥重要作用。为了研究SLP-2基因在肿瘤里的功能，将人类SLP-2基因全长编码区定向连入pEGFP—C3质粒，使SLP-2蛋白可以与绿色荧光蛋白在乳腺癌细胞MCF-7内融合表达。而且细胞荧光实验发现SLP-2蛋白主要定位在MCF-7细胞的胞质内，这为进一步研究SLP-2基因在乳腺癌中的功能奠定了实验基础。
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| 关 键 词： | SLP-2 重组质粒 融合蛋白表达 细胞定位 |
Construction of pEGFP-C3-SLP-2 Plasmid and its Expression and Localization in MCF-7 Cells |
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| WANG Yi＇-jun,WU Wen-tao,XU Tian,XIANG Shuang-lin,ZHANG Jian,HAN Mei.Construction of pEGFP-C3-SLP-2 Plasmid and its Expression and Localization in MCF-7 Cells[J].ACTA Laser Biology Sinica,2010,19(1):10-13,24. |
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| Authors: | WANG Yi＇-jun WU Wen-tao XU Tian XIANG Shuang-lin ZHANG Jian HAN Mei |
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| Institution: | (College of life science, Hunan Normal University, Changsha 410081, Hunan, China) |
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| Abstract: | Human stomatin like protein 2 （ SLP-2 ） is a novel cancer-related gene. Its protein is overexpressed in many human cancers, such as esophageal squamous cell carcinoma and lung cancer. In Breast cancer, high-level SLP-2 expression were associated with significantly decreased survival. To study the function of SLP-2 in these cancers, the coding region of human SLP-2 gene was successfully subcloned into the eukaryotic green fluorescent protein（GFP） expressing recombinant plasmids, pEGFP-C3. The vector was then transfected into MCF-7 cells for efficient expression. Moreover, results from fluorescent observation shows that GFP-SLP-2 mainly localized in cytoplasma, thus providing a useful clue for further research on the function of SLP-2 in breast cancer. |
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| Keywords: | SLP-2 SLP-2 recombinant plasmid fusion protein expression cell localization |
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