Wheat chromosome 2D carries genes controlling the activity of two DNA-degrading enzymes |
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Authors: | Y Yen P S Baenziger |
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Institution: | (1) Department of Agronomy, University of Nebraska, P.O. Box 830915, 68583-0915 Lincoln, NE, USA |
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Abstract: | DNA-degrading enzymes of 24.0 kDa and 27.0 kDa were observed to have different activities in two common wheat (Triticum aestivum L.) cultivars, Wichita and Cheyenne . A substrate-based SDS-PAGE assay revealed that these two enzymes were much more active in Wichita than in Cheyenne . Genes controlling the activities of these two enzymes were localized on chromosome 2D by testing DNA-degrading activities in reciprocal chromosome substitution lines between Wichita and Cheyenne . While the allele on Wichita chromosome 2D stimulated the activities of the 24.0- and 27.0-kDa enzymes in Cheyenne, the allele on Cheyenne chromosome 2D did not reduce the activities of the 24-kDa and 27-kDa enzymes in Wichita . Whether these genes code for the DNA-degrading enzymes themselves or for factors that regulate the enzyme activities remains unknown.This work was supported in part by USDA-Competitive Research Grants Office grant No. 90-37140-5426 to P.S.B. Contribution from Agricultural Research Division, University of Nebraska. Journal Series Number 10304 |
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Keywords: | Triticum aestivum DNase Nuclease Cytogenetics Gene mapping |
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