Isolation of plasma membranes from Xenopus embryos |
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Authors: | Günther Bretzel Jutta Janeczek Jochen Born Manorama John Heinz Tiedemann Hildegard Tiedemann |
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Affiliation: | (1) Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000 Berlin 33 |
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Abstract: | Summary Plasma membranes were isolated in high yield from Xenopus gastrulae by repeated sedimentation in discontinuous sucrose gradients. Most of the yolk was separated by lowspeed sedimentation before centrifugation on the discontinuous sucrose gradients. The isolation of plasma membranes was followed by covalent labelling of the surface of dissociated gastrula cells with diazoniobenzene sulphonate, by electron microscopy and the distribution of enzymatic markers. The isolated plasma membranes have a low neural inducing activity as compared to other cell constituents. |
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Keywords: | Plasma membranes Amphibian embryogenesis Neural induction Xenopus laevis |
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