Amino acid sequences of ovomucoid third domains from 27 additional species of birds |
| |
Authors: | Izydor Apostol Anthony Giletto Tomoko Komiyama WenLei Zhang and Michael Laskowski Jr |
| |
Institution: | (1) Department of Chemistry, Purdue University, 47907 West Lafayette, Indiana;(2) Present address: Somatogen, Inc., 5797 Central Ave., 80301 Boulder, Colorado;(3) Present address: Department of Pathology, Duke University Medical Center, 27710 Durham, North Carolina |
| |
Abstract: | Ovomucoids consist of a single polypeptide chain which is composed of three tandem Kazal domains. Each Kazal domain is an actual or putative protein inhibitor of serine proteinases. Ovomucoid third domains were already isolated and sequenced from 126 species of birds (Laskowskiet al., 1987, 1990). This paper adds 27 new species. A number of generalizations are made on the basis of sequences from 153 species. The residues that are in contact with the enzyme in enzyme-inhibitor complexes are strikingly hypervariable. While the primary specificity residue,P
1, is the most variable; substitutions occur predominantly among aliphatic, hydrophobic residues. Consensus sequences for an avian ovomucoid third domain, for a b-type Kazal domain (i.e., a COOH terminal domain of multidomain inhibitors) and for a general Kazal domain are given. Finally, the individual new sequences are briefly discussed. |
| |
Keywords: | Ovomucoid third domain sequences protein proteinase inhibitors evolution of birds |
本文献已被 SpringerLink 等数据库收录! |
|