Reactive oxygen-induced reactive oxygen formation during human sperm capacitation |
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| Authors: | Eve de Lamirande Geneviève Lamothe |
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| Affiliation: | 1. Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia;2. Department of Medical Physics and Bioengineering, Institute of Physics, Nanotechnology and Telecommunications, St. Petersburg State Polytechnical University, Polytechnicheskaya st. 29, St. Petersburg 195251, Russia;1. SaBio IREC (CSIC–UCLM–JCCM), Campus Universitario, Albacete, Spain;2. Regional Center of Animal Selection and Reproduction (CERSYRA) JCCM, Valdepeñas, Spain;3. Department of Molecular Biology, University of León, León, Spain;4. Institute for Animal Health and Cattle Development (INDEGSAL), University of León, León, Spain;5. Faculty of Pharmacy (UCLM), Albacete, Spain;1. Centro Nacional Patagónico, Bvd. Brown 2915, U9120ACD, Puerto Madryn, Argentina;2. Universidad Nacional de la Patagonia San Juan Bosco, Bvd. Brown 3051, U9120ACD, Puerto Madryn, Argentina;1. Department of Obstetrics and Gynecology, Third Affiliated Hospital of Guangzhou Medical University, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, PR China;2. Department of Obstetrics and Gynecology, Southern Medical University, Guangzhou, PR China;1. Iuliu Ha?ieganu University of Medicine and Pharmacy, Epidemiology and Primary Health Care Department, 8th Victor Babes Street, 400012 Cluj–Napoca, Romania;2. Centre Hospitalier Universitaire Charles Nicolle, Département d’épidémiologie et de santé publique, 1, rue de Germont, 76000 Rouen cedex 1, France |
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| Abstract: | Physiological processes are often activated by reactive oxygen species (ROS), such as the superoxide anion (O2 –) and nitric oxide (NO) produced by cells. We studied the interactions between NO and O2–, and their generators (NO synthase, NOS, and a still elusive oxidase), in human spermatozoa during capacitation (transformations needed for acquisition of fertility). Albumin, fetal cord serum ultrafiltrate, and L-arginine triggered capacitation and ROS generation (NO and O2–) and superoxide dismutase (SOD) and NOS inhibitors prevented all these effects. Surprisingly, capacitation due to exogenous NO (or O2–) was also blocked by SOD (or NOS inhibitors). Probes used were proven specific and innocuous on spermatozoa. Whereas O2– was needed only for 30 min, the continuous NO generation was essential for hours. Capacitation caused a time-dependent increase in protein tyrosine nitration that was prevented by SOD and NOS inhibitors, suggesting that O2– and NO· also act via the formation of ONOO–. Spermatozoa treated with NO (or O2–) initiated a dose-dependent O2– (or NO) production, providing, for the first time in cells, a strong evidence for a two-sided ROS-induced ROS generation. Data presented show a close interaction between NO and O2– and their generators during sperm capacitation. |
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