| 人鼠肝组织嵌合体模型的制备 |
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| 引用本文： | 刘光泽,周笛,范沛,顾为望.人鼠肝组织嵌合体模型的制备[J].中国实验动物学杂志,2010(10):55-57,90. |
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| 作者姓名： | 刘光泽 周笛 范沛 顾为望 |
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| 作者单位： | [1]解放军第四五八医院全军肝病中心,广州510602 [2]南方医科大学比较医学研究所,广州501515 |
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| 基金项目： | 广东省科技项目（项目编号A060305004） |
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| 摘 要： | 目的研究制备人鼠肝组织嵌合小鼠模型。方法将人骨髓干细胞直接注射到一定日龄胎鼠肝组织,每只注射移植约1×109人骨髓干细胞。用免疫组化对出生一定日龄移植小鼠肝脏进行甲胎蛋白免疫组织化学检测,检定分析人肝细胞在小鼠体内嵌合生长情况。结果移植人骨髓干细胞胎鼠出生2月龄、12月龄可检测到甲胎蛋白。结论将人骨髓干细胞移植小鼠肝脏内能够存活并分化成人肝细胞并能够长期存活。
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| 关 键 词： | 人鼠嵌合 甲胎蛋白 |
The Preliminary Investigation of Human-mouse Liver Chimeric Model Preparation |
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| LIU Guang-ze,ZHOU Di,FAN Pei,GU Wei-wang.The Preliminary Investigation of Human-mouse Liver Chimeric Model Preparation[J].Chinese Journal of Laboratory Animal Science,2010(10):55-57,90. |
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| Authors: | LIU Guang-ze ZHOU Di FAN Pei GU Wei-wang |
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| Institution: | 1. The Liver Disease Centre of PLA,458 th Hospital of PLA,Guangzhou 510602,China; 2. The Institute of Comparative Medicine of South Medical University,Guangzhou 510515,China) |
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| Abstract: | Objective To establish the human mouse chimeric model of HCV. Methods The human bone marrow stem cells were transplanted into each mouse with the number of 1 × 109 via liver to fetal mouse in KM mouse. Testing the AFP in the transplanted mouse liver by immunohistochemistry to analyze the living condition of human liver cell in the mouse body. Results AFP was tested after the transplantation of months within its normal value. Conclusion The human bone marrow stem cells can live and differentiate into the liver cells after transplantation into mouse liver. |
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| Keywords: | Human-mouse chimeric AFP |
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