Reaggregation of fetal rat brain cells in a stationary culture system II: Ultrastructural characterization |
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Authors: | Rolf Bjerkvig |
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Institution: | (1) The Gade Institute, Department of Pathology, University of Bergen, N-5016 Haukeland Hospital, Bergen, Norway |
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Abstract: | Summary Ultrastructural characteristics of fetal rat brain cell aggregates in a three-dimensional stationary culture system are described.
Transmission electron microscopy showed immature cells which developed into mature astrocytes, oligodendrocytes, and neurons
during 20 d in culture. This was accompanied by the development of a neuropil where myelinated axons and synaptic complexes
were observed. In addition to confirming earlier ultrastructural investigations on fetal rat brain cell aggregates, the stationary
culture system also showed the presence of histiotypic regions within the aggregates. These regions consisted of ependymal
cells where cilia were observed on the cell surfaces. Structures resembling subependymal basement membrane labyrinths were
also observed. Macrophages seemed to be more numerous in the stationary cultures as compared to other culture systems. The
stationary culture system may provide aggregates that are ultrastructurally more complex than those obtained by rotation mediated
systems.
This investigation was supported by The Norwegian Cancer Society. |
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Keywords: | brain aggregates ultrastructure |
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