Coriaria Lactone Increased the Intracellular Level of Calcium through the Voltage-gated Calcium Channels in Rat Hippocampal Neurons |
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Authors: | Qin Zhang Xiaohui Lai Daqing Liao Hermann Stefan Dong Zhou |
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Affiliation: | (1) Department of Neurology, West China Hospital, Sichuan University, 610041 Chengdu, Sichuan, People’s Republic of China;(2) Laboratory of Anesthesiology and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, People’s Republic of China;(3) Department of Neurology, Epilepsy Center Erlangen, University of Erlangen-Nuremberg, Erlangen, Germany |
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Abstract: | To investigate the effects of Coriaria Lactone (CL), an epileptogenic substance, on intracellular levels of calcium ([Ca2+]i) and physiological properties of voltage-gated calcium channels (VGCCs). Ratiometric calcium imaging using Fura Red and whole-cell voltage patch-clamp technique were explored on freshly isolated rat hippocampal neurons exposed to CL. Coriaria Lactone increased [Ca2+]i from 118 ± 21 to 440 ± 35 nM; VGCCs and calcium influx through NMDA receptor served as the main routes of entry. Coriaria Lactone could enhance both Low voltage activated (LVA) and High voltage activated calcium currents in a concentration-dependent way, and its effect on LVA current was more potent (about 60%). The increased calcium currents were accompanied by the shift of voltage-dependent steady-state inactivation to more positive potentials. These effects of CL, especially its impact on LVA current, could activate different calcium-dependent signaling pathways, and influence cellular excitable properties as well, which might play an important role in CL’s epileptogenic process. Q. Zhang and X. Lai contributed equally to this article. |
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Keywords: | Coriaria Lactone Calcium Voltage-gated calcium channel Hippocampal neurons Epilepsy |
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