Influence of an estrone-desulfating intestinal flora on the enterohepatic circulation of estrone-sulfate in rats |
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| Authors: | J van Eldere G Parmentier J Robben H Eyssen |
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| Institution: | 1. Department of Laboratory Diagnostics, University Medical Centre Maribor, Maribor, Slovenia;2. Department of Laboratory Medicine, Cliniques Universitaires St-Luc and Universite Catholique de Lovain, Brussels, Belgium;1. Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan;2. Department of Systems Aging Science and Medicine, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan;1. Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA;2. Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA;3. Genentech, South San Francisco, CA 94080, USA;4. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;5. Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA;6. BASE Initiative, Betty Irene Moore Children’s Heart Center, Lucile Packard Children’s Hospital, Stanford University School of Medicine, Stanford, CA 94304, USA;7. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA |
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| Abstract: | The fecal and urinary excretion of orally administered 4-14C]estrone-3-sulfate was studied in germfree (GF) rats, conventional (CV) rats and gnotobiotic rats selectively associated with estrone-desulfating and/or cecal-volume reducing microorganisms. The time required to excrete 50% of the total label recovered (t 1/2) was 22 h in CV rats vs 32 h in GF rats. Gnotobiotic rats selectively associated with a cecal volume-reducing flora (CRF rats) excreted the label even faster (t 1/2 = 13 h) than CV rats. Association of GF rats as well as CRF rats with estrone-desulfating microorganisms (termed S1 + S2 + R9 rats and CRF + S1 + S2 + R9 rats, respectively) led to a slower excretion of labeled products (t 1/2 = 38 h in S1 + S2 + R9 rats and t 1/2 = 27 h in CFR + S1 + S2 + R9 rats). Intestinal microbial desulfation also increased the relative part of the urinary excretion from 4% in GF rats to 8% in S1 + S2 + R9 rats and from 3% in CRF rats to 9% in CFR + S1 + S2 + R9 rats. We conclude that intestinal microbial desulfation enhances the enterohepatic circulation of orally administered estrone-3-sulfate. |
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