A specific and inexpensive assay of radiolabeled long-chain acyl-coenzyme A in isolated hepatocytes. |
| |
| Authors: | H K Stals P E Declercq |
| |
| Institution: | Katholieke Universiteit Leuven, Instituut voor Farmaceutische Wetenschappen, Belgium. |
| |
| Abstract: | A simple procedure for determining radiolabeled long-chain acyl-CoA levels in isolated rat hepatocytes has been developed. It consists of a classical extraction of long-chain acyl-CoAs after preliminary removal of the excess labeled free fatty acid. A two-step TLC purification ensures elimination of long-chain acylcarnitine, the main interferent in most methods, and other common lipids. The purity of the acyl-CoA was confirmed by a second TLC system and by spectral analysis. Overall recovery was approximately 70%. |
| |
| Keywords: | |
|
|
