Detection ofDesulfobacter in oil field environments by non-radioactive DNA probes |
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| Authors: | D. E. Brink I. Vance D. C. White |
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| Affiliation: | (1) BP America Inc., Warrensville Research Center, 4440 Warrensville Center Road, 44128-2837 Cleveland, Ohio, USA;(2) Center for Environmental Biotechnology, 10515 Research Drive, 37932-2575 Knoxville, Tennessee, USA;(3) Present address: BP Exploration Prudhoe Laboratory (B5), P.O. Box 196612, 99519-6612 Anchorage, AK, USA;(4) Present address: BP Research & Engineering Centre, Chertsey Road, TW16 7LN Sunbury-on-Thames, Middlesex, UK |
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| Abstract: | Acetate-utilizing, sulfate-reducing bacteria of the genusDesulfobacter were enriched from an oilfield seawater-injection system. These bacteria were detected in enrichment culture and verified asDesulfobacter by an oligonucleotide DNA probe that targets aDesulfobacter-specific sequence of ribosomal RNA. TheseDesulfobacter produced extensive biofilm in culture and exhibited high levels of hydrogenase activity, which suggests a sessile habit and a role in the cathodic depolarization mechanism of microbially influenced corrosion. Phospholipid analyses indicated that one of the oilfield isolates ofDesulfobacter lacked the fatty acid 10 Me 16:0, which has been used as a biomarker for this genus. Desulfobacter were infrequently encountered in the oilfield systems studied, and they constituted a small portion of the total microflora at any sampling location. |
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