Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction |
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Authors: | Nicolás M Cecchini DeQuantarius J Speed Suruchi Roychoudhry Jean T Greenberg |
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Institution: | 1. Department of Molecular Genetics and Cell Biology, The University of Chicago, 929 East 57th Street GCIS 524W, Chicago, IL, 60637 USA;2. Department of Molecular Genetics and Cell Biology, The University of Chicago, 929 East 57th Street GCIS 524W, Chicago, IL, 60637 USA
Centre for Plant Sciences, University of Leeds, Leeds, LS2 9JT UK |
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Abstract: | The proper subcellular localization of defense factors is an important part of the plant immune system. A key component for systemic resistance, lipid transfer protein (LTP)-like AZI1, is needed for the systemic movement of the priming signal azelaic acid (AZA) and a pool of AZI1 exists at the site of AZA production, the plastid envelope. Moreover, after systemic defense-triggering infections, the proportion of AZI1 localized to plastids increases. However, AZI1 does not possess a classical plastid transit peptide that can explain its localization. Instead, AZI1 uses a bipartite N-terminal signature that allows for its plastid targeting. Furthermore, the kinases MPK3 and MPK6, associated with systemic immunity, promote the accumulation of AZI1 at plastids during priming induction. Our results indicate the existence of a mode of plastid targeting possibly related to defense responses. |
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Keywords: | Systemic immunity Arabidopsis thaliana signal-anchored proteins defense priming lipid transfer protein AZI1 |
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