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Genetic diversity of tomato response to heat stress at the QTL and transcriptome levels |
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| Authors: | Estelle Bineau Isidore Diouf Yolande Carretero Renaud Duboscq Frédérique Bitton Anis Djari Mohamed Zouine Mathilde Causse |
| Affiliation: | 1. Génétique et Amélioration des Fruits et Légumes, Centre de Recherche PACA, INRAE, UR1052, Domaine Saint Maurice, 67 Allée des Chênes, CS60094, Montfavet, 84143 France GAUTIER Semences, route d'Avignon, Eyragues, 13630 France These authors contributed equally to this study.;2. Génétique et Amélioration des Fruits et Légumes, Centre de Recherche PACA, INRAE, UR1052, Domaine Saint Maurice, 67 Allée des Chênes, CS60094, Montfavet, 84143 France These authors contributed equally to this study.;3. Génétique et Amélioration des Fruits et Légumes, Centre de Recherche PACA, INRAE, UR1052, Domaine Saint Maurice, 67 Allée des Chênes, CS60094, Montfavet, 84143 France;4. Laboratory of Genomics and Biotechnology of Fruit, University of Toulouse, INPT, Avenue de l'Agrobiopole BP 32607, Castanet-Tolosan, F-31326 France UMR990 Génomique et Biotechnologie des Fruits, INRAE, Chemin de Borde Rouge, Castanet-Tolosan, F-31326 France |
| Abstract: | Tomato is a widely cultivated crop, which can grow in many environments. However, temperature above 30°C impairs its reproduction, subsequently impacting fruit yield. We assessed the impact of high-temperature stress (HS) in two tomato experimental populations, a multi-parental advanced generation intercross (MAGIC) population and a core-collection (CC) of small-fruited tomato accessions. Both populations were evaluated for 11 traits related to yield components, phenology and fruit quality in optimal and HS conditions. HS significantly impacted all traits in both populations, but a few genotypes with stable yield under HS were identified. A plasticity index was computed for each individual to measure the extent of the heat impact for each trait. Quantitative trait loci (QTL) were detected in control and HS conditions as well as for plasticity index. Linkage and genome-wide association analyses in the MAGIC and CC populations identified a total of 98 and 166 QTLs, respectively. Taking the two populations together, 69 plasticity QTLs (pQTLs) were involved in tomato heat response for 11 traits. The transcriptome changes in the ovary of six genotypes with contrasted responses to HS were studied, and 837 genes differentially expressed according to the conditions were detected. Combined with previous transcriptome studies, these results were used to propose candidate genes for HS response QTLs. |
| Keywords: | tomato Solanum lycopersicum L. heat stress phenotypic plasticity transcriptome quantitative trait loci genome-wide association study |