Increased copy number of gibberellin 2-oxidase 8 genes reduced trailing growth and shoot length during soybean domestication |
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| Authors: | Xin Wang Man-Wah Li Fuk-Ling Wong Ching-Yee Luk Claire Yik-Lok Chung Wai-Shing Yung Zhili Wang Min Xie Shikui Song Gyuhwa Chung Ting-Fung Chan Hon-Ming Lam |
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| Institution: | 1. School of Life Sciences and the Centre for Soybean Research of the State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong, China;2. FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China;3. Department of Biotechnology, Chonnam National University, Yeosu, South Korea |
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| Abstract: | Copy number variations (CNVs) play important roles in crop domestication. However, there is only very limited information on the involvement of CNVs in soybean domestication. Trailing growth and long shoots are soybean adaptations for natural habitats but cause lodging that hampers yield in cultivation. Previous studies have focused on Dt1/2 affecting the indeterminate/determinate growth habit, whereas the possible role of the gibberellin pathway remained unclear. In the present study, quantitative trait locus (QTL) mapping of a recombinant inbred population of 460 lines revealed a trailing-growth-and-shoot-length QTL. A CNV region within this QTL was identified, featuring the apical bud-expressed gibberellin 2-oxidase 8A/B, the copy numbers of which were positively correlated with expression levels and negatively with trailing growth and shoot length, and their effects were demonstrated by transgenic soybean and Arabidopsis thaliana. Based on the fixation index, this CNV region underwent intense selection during the initial domestication process. |
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| Keywords: | domestication gene copy number shoot length growth habit gibberellin 2-oxdase soybean |
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