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Evolutionary trade-offs at the Arabidopsis WRR4A resistance locus underpin alternate Albugo candida race recognition specificities |
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| Authors: | Baptiste Castel Sebastian Fairhead Oliver J. Furzer Amey Redkar Shanshan Wang Volkan Cevik Eric B. Holub Jonathan D. G. Jones |
| Affiliation: | 1. The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, NR4 7UH, Norwich United Kingdom;2. The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, NR4 7UH, Norwich United Kingdom Warwick Crop Centre, School of Life Sciences, University of Warwick, CV35 9EF, Wellesbourne United Kingdom;3. The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, NR4 7UH, Norwich United Kingdom Department of Biology, University of North Carolina, Chapel Hill, NC, 27599 USA;4. The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, NR4 7UH, Norwich United Kingdom Department of Genetics, University of Cordoba, 14071, Cordoba Spain;5. The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, NR4 7UH, Norwich United Kingdom Department of Biology and Biochemistry, The Milner Centre for Evolution, University of Bath, BA2 7AY, Bath United Kingdom;6. Warwick Crop Centre, School of Life Sciences, University of Warwick, CV35 9EF, Wellesbourne United Kingdom |
| Abstract: | The oomycete Albugo candida causes white rust of Brassicaceae, including vegetable and oilseed crops, and wild relatives such as Arabidopsis thaliana. Novel White Rust Resistance (WRR) genes from Arabidopsis enable new insights into plant/parasite co-evolution. WRR4A from Arabidopsis accession Columbia (Col-0) provides resistance to many but not all white rust races, and encodes a nucleotide-binding, leucine-rich repeat immune receptor. Col-0 WRR4A resistance is broken by AcEx1, an isolate of A. candida. We identified an allele of WRR4A in Arabidopsis accession Øystese-0 (Oy-0) and other accessions that confers full resistance to AcEx1. WRR4AOy-0 carries a C-terminal extension required for recognition of AcEx1, but reduces recognition of several effectors recognized by the WRR4ACol-0 allele. WRR4AOy-0 confers full resistance to AcEx1 when expressed in the oilseed crop Camelina sativa. |
| Keywords: | immunity resistance gene NLR natural variation evolution effector recognition crop protection Arabidopsis thaliana camelina |