Non-lactose-fermenting uropathogenic Escherichia coli from dogs: virulence profile characterization |
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| Authors: | F.M. Siqueira S. De Carli C.E. Lopes L. Machado T.R. Vieira Á.G. Pöppl M.R.I. Cardoso A. Zaha |
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| Affiliation: | 1. Laboratory of Veterinary Bacteriology, Faculty of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil;2. Veterinary Clinical Hospital, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil;3. Laboratory of Veterinary Preventive Medicine, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil;4. Center of Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil |
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| Abstract: | Non-lactose-fermenting Escherichia coli (NLFEC) has a few descriptive studies restricted to human infections. In the present study, isolates of NLFEC obtained from urine samples of dogs with hyperadrenocorticism were characterized regarding their virulence ability, biofilm formation capacity and antimicrobial susceptibility profile. Escherichia coli lactose-fermenting strains from urinary infection in dogs with the same conditions were analysed to provide comparisons. The non-lactose-fermenting E. coli strains were classified as belonging to clade I E. coli, whereas the lactose-fermenting strains were classified in phylogroup B2. All strains presented virulence markers to adhesion, iron acquisition, toxins, colicin and cytotoxin production, and biofilm regulation. Components of the extracellular matrix in addition to the in vitro biofilm formation ability were observed in the strains. Multidrug resistance (MDR) profiles were observed by in vitro susceptibility tests to all NLFEC strains. In summary, non-lactose-fermenting uropathogenic E. coli from dogs behaves similar to lactose-fermenting E. coli, exhibiting MDR profile, and pathogenic potential of promote animal infections. |
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| Keywords: | antibiotic resistance canine cystitis non-lactose-fermenting E. coli phylogenetic classification uropathogenic Escherichia coli |
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