Effect of near-infrared and blue laser light on vero E6 cells SARS-CoV-2 infection model |
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| Authors: | Luisa Zupin Libera Clemente Francesco Fontana Sergio Crovella |
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| Affiliation: | 1. Institute for Maternal and Child Health, IRCCS “Burlo Garofolo”, Trieste, Italy;2. Division of Laboratory Medicine, University Hospital Giuliano Isontina (ASU GI), Trieste, Italy;3. Department of Biological and Environmental Sciences, College of Arts and Sciences, University of Qatar, Doha, Qatar |
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| Abstract: | Photobiomodulation therapy (PBMT) employing laser light has been emerging as a safe strategy to challenge viruses. In this study the effect of blue and near-infrared (NIR) laser light was assessed in an in vitro model of SARS-CoV-2 infection. PBMT at blue wavelength inhibited viral amplification when the virus was directly irradiated and then transferred to cell culture and when cells already infected were treated. The NIR wavelength resulted less efficacious showing a minor effect on the reduction of the viral load. The cells receiving the irradiated virus or directly irradiated rescued their viability to level comparable to not treated cells. Virion integrity and antigenicity were preserved after blue and NIR irradiation, suggesting that the PBMT antiviral effect was not correlated to viral lipidic envelope disruption. Our results suggested that PBMT can be considered a valid strategy to counteract SARS-CoV-2 infection, at least in vitro. |
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| Keywords: | antiviral blue and near-infrared laser light photobiomodulation therapy SARS-CoV-2 vero E6 cells |
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