Specific labeling and identification of bacteria based on concentration-dependent carbon dot staining combined with hyperspectral imaging |
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| Authors: | Sifan Lin Ze Ke Mingwei Lu Yanzhong Zhou Wenrui Tang Siqi Zhu Yongqiang Zhang Zhen Li Hao Yin Zhenqiang Chen |
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| Institution: | 1. Department of Optoelectronic Engineering, Jinan University, Guangzhou, China;2. Guangdong Provincial Engineering Research Center of Crystal and Laser Technology, Guangzhou, China;3. Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou, China |
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| Abstract: | Concentration-dependent carbon dot (CD) fluorescence was developed and utilized alongside hyperspectral microscopy as a specific labeling and identification technique for bacteria. Staining revealed that the CD concentration within cells depended on the characteristic intracellular environment of the species. Therefore, based on the concentration dependence of the CD fluorescence, different bacterial species were specifically labeled. Hyperspectral microscopy captured subtle fluorescence variations to identify bacteria. Method validation using Bacillus subtilis and Bacillus licheniformis succeeded with an identification accuracy of 99%. As a simple, rapid method for labeling and identifying bacterial species in mixtures, this technique has excellent potential for bacterial community studies. |
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| Keywords: | bacteria concentration-dependent carbon dots hyperspectral microscopy specific labeling |
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