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Comparative analysis of dermal collagen and lipids in cereblon ablated mice using a multimodal nonlinear optical system |
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| Authors: | Chang Woo Park Seungje Jeon Seong-Hoon Kwon Jun-Hyung Jung Jung Eun Seol Chul-Seung Park Steve K. Cho Do-Kyeong Ko |
| Affiliation: | 1. Department of Physics and Photon Science, Gwangju Institute of Science and Technology, Gwangju, South Korea;2. School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, South Korea Smart Marine Therapeutics Center, Cardiovascular and Metabolic Disease Center, Inje University, Busan, South Korea;3. Pohang Accelerator Laboratory, Pohang, Gyeongbuk, South Korea;4. School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, South Korea;5. Smart Marine Therapeutics Center, Cardiovascular and Metabolic Disease Center, Inje University, Busan, South Korea Department of Dermatology, Inje University Busan Paik Hospital, Inje University, Busan, South Korea |
| Abstract: | By utilizing a multimodal nonlinear optical system that combines coherent anti-Stokes Raman scattering and second harmonic generation to investigate biological characteristics of dermal tissues ex vivo, we demonstrate the potential feasibility of using this optical approach as a powerful new investigative tool for future biomedical research. For this study, our optical system was utilized for the first time to analyze lipid and collagen profiles in cereblon knockout (KO) mouse skin, and we were able to discover significant alterations in the number of carbon–carbon double bonds (wild-type vs. cereblon KO; NCC: 0.75 vs. 0.85) of skin fatty acids in triacylglycerides as well as changes in dermal collagen fibers (25% reduction in cereblon KO). By adopting our optical system to biological studies, we provide researchers with another diagnostic approach to validate their experimental results, which will significantly advance the state of biomedical research. |
| Keywords: | coherent anti-stokes Raman scattering spectroscopy cereblon lipid phase retrieval second harmonic generation |