|
|||||
|
|
Studying the genotoxic effects of high intensity terahertz radiation on fibroblasts and CNS tumor cells |
|
| Authors: | Dmitry S Sitnikov Veronika A Revkova Inna V Ilina Svetlana A Gurova Pavel S Komarov Evgenia V Struleva Mikhail A Konoplyannikov Vladimir A Kalsin Vladimir P Baklaushev |
| Institution: | 1. Joint Institute for High Temperatures of the Russian Academy of Sciences, Moscow, Russia;2. Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies FMBA of Russia, Moscow, Russia
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia;3. Obninsk Institute for Nuclear Power Engineering, National Research Nuclear University MEPhI, Obninsk, Russia;4. Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies FMBA of Russia, Moscow, Russia Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, Moscow, Russia |
| Abstract: | The data is obtained on the effect of high-intensity pulses of terahertz (THz) radiation with a broad spectrum (0.2–3 THz) on cell cultures. We have evaluated the threshold exposure parameters of THz radiation causing genotoxic effects in fibroblasts. Phosphorylation of histone H2AX at Ser 139 (γH2AX) was chosen as a marker for genotoxicity and a quantitative estimation of γH2AX foci number in fibroblasts was performed after cell irradiation with THz pulses for 30 min. No genotoxic effects of THz radiation were observed in fibroblasts unless peak intensity and electric field strength exceeded 21 GW cm?2 and 2.8 MV cm?1, respectively. In tumor cell lines (neuroblastoma (SK-N-BE (2)) and glioblastoma (U87)), exposure to THz pulses with peak intensity of 21 GW cm?2 for 30 min caused no morphological changes as well as no statistically significant increase in histone phosphorylation foci number. |
| Keywords: | fibroblasts genotoxic effects glioblastoma neuroblastoma terahertz radiation γH2AX |