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Metabolic engineering challenges of extending N-glycan pathways in Chinese hamster ovary cells
Institution:1. Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA;2. Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA;1. Department of Biological Engineering, Inha University, 100 Inha-ro, Michuhol-gu, Incheon 22212, Republic of Korea;2. Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea;3. Department of Global Innovative Drug, The Graduate School of Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea;4. Department of Life Sciences, Department of Health Sciences and Technology, GAIHST, Gachon University, Seongnam-daero, Sujeong-gu, Seongnam 13120, Republic of Korea;1. Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA;2. GlycoBac, LLC, Laramie, WY 82072, USA;3. Institute of Biological Chemistry, Academia Sinica 128, Nankang, Taipei 115, Taiwan;1. Biomedical Engineering and Biotechnology, University of Massachusetts Lowell, Lowell, MA 01850, USA;2. Division of Biotechnology Review and Research II, Office of Biotechnology Products, OPQ, CDER, FDA, Silver Spring, MD, USA;3. Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive, Singapore 117585, Singapore;1. Biologics Upstream Process Development, Merck & Co., Inc., 2000 Galloping Hill Road, Kenilworth, NJ, 07033, USA;2. Genetics and Pharmacogenomics, Merck & Co., Inc., 2000 Galloping Hill Road, Kenilworth, NJ, 07033, USA
Abstract:
Keywords:N-acetyllactosamine (LacNAc)  β-1  3-N-Acetylglucosaminyltransferase 2 (B3GNT2)  β-1  4-Galactosyltransferase 1 (B4GALT1)  α-2  3-Sialyltransferase  Golgi  Glycosylation
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