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Esterase D interacts with metallothionein 2A and inhibits the migration of A549 lung cancer cells in vitro |
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| Authors: | Wen Yao Xinpeng Chen Xiaoling Cui Bangzhao Zhou Baoxiang Zhao ZhaoMin Lin Junying Miao |
| Affiliation: | 1. Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Qingdao, People's Republic of China Jinan Microecological Biomedicine Shandong Laboratory, Jinan, People's Republic of China;2. Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Qingdao, People's Republic of China Hubei Key laboratory of Edible Wild Plants Conservation & Utilization, 3. School of Life Science, 4. National Demonstration Center for Experimental Biology Education, Hubei Normal University, Huangshi, People's Republic of China;5. Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Qingdao, People's Republic of China;6. School of Chemistry and Chemical Engineering, Institute of Organic Chemistry, Shandong University, Jinan, People's Republic of China;7. Institute of Medical Science, The Second Hospital of Shandong University, Jinan, People's Republic of China |
| Abstract: | Esterase D (ESD) is a nonspecific esterase widely distributed in various organisms. ESD plays an important role in regulating cholesterol efflux, inhibiting viral replication and lung cancer growth. MT2A (metallothionein 2A) is the most important isoform of metallothionein (MTs) in human and high expression of MT2A in tumors represents poor prognosis and metastatic behavior. However, there are no reports about the molecular mechanism of ESD in the regulation of tumor metastasis. In this study, we found for the first time that activation ESD promoted its interaction with MT2A and decreased the protein level of MT2A, which resulting in the concentration of free zinc ions up-regulated, and inhibited the migration of A549 lung cancer cells in vitro. |
| Keywords: | A549 lung cancer cells Esterase D metallothionein 2A migration |