In vitro characterization of estrogen induced syrian hamster renal tumors: Comparison with an immortalized cell line derived from diethylstilbestrol-treated adult hamster kidney |
| |
Authors: | Alfonso Gonzalez Terry D. Oberley Janice L. Schultz Jennifer Ostrom Jonathan J. Li |
| |
Affiliation: | (1) Department of Pathology, University of Utah School of Medicine, and Laboratory Service, Veterans Affairs Medical Center, 84148 Salt Lake City, Utah;(2) Department of Pathology, University of Wisconsin Medical School, 53706 Madison, Wisconsin;(3) Hormonal Carcinogenesis Laboratory, Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, 99164 Pullman, Washington;(4) Pathology Section, William S. Middleton Memorial Veterans Hospital, 2500 Overlook Terrace, 53705 Madison, WI |
| |
Abstract: | Summary Primary diethylstilbestrol-induced kidney tumors from Syrian hamsters were grown in vitro and maintained in culture for 6 mo. Combined immunohistochemical studies using antibodies to intermediate filaments and ultrastructural studies of tumor cells in culture exhibited characteristics similar to tumor cells in vivo. Furthermore, the cells manifested transformed properties in culture; they grew both as multilayered colonies attached to the tissue culture substrate and as floating multicellular colonies (spheroids). When cultured cells were injected into diethylstilbestrol-treated recipient hamsters, tumors developed at the injection sites. In contrast, renal tubules or whole kidney cortex from control hamsters cultured in the same medium underwent only short-term growth, with senescence developing after approximately 1 mo. However, cell cultures of kidney cortex from animals treated in vivo for 5 mo. with diethylstilbestrol formed a cell line. This diethylstilbestrol-induced cell line has been maintained in culture for 1.5 yr and has the following characteristics: a) it is anchorage-dependent, b) it is negative in in vivo tumorigenicity tests, and c) cultured cells are histochemically and ultrastructurally similar to cultured tumor cells. This culture system should prove to be of use in studying hormonal carcinogenesis in vitro. This study was supported by the Medical Research Service, Department of Veterans Affairs, Washington, DC, and by grant CA-22008 from the National Cancer Institute, NIH, DHHS, Bethesda, MD. |
| |
Keywords: | cell culture hamster renal tumor |
本文献已被 SpringerLink 等数据库收录! |
|