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Demonstration of rod and cone photoreceptors in the lamprey retina by freeze-replication and immunofluorescence
Authors:Makoto Ishikawa  Masashi Takao  Hiroshi Washioka  Fumio Tokunaga  Hiroshi Watanabe  Akira Tonosaki MD
Affiliation:(1) Departments of Anatomy, Yamagata University School of Medicine, Yamagata;(2) Departments of Otolaryngology, Yamagata University School of Medicine, Yamagata;(3) Department of Biophysics, Faculty of Science, Tohoku University, Sendai, Japan;(4) Department of Anatomy, Yamagata University School of Medicine, 990-23 Yamagata, Japan
Abstract:Summary In common with other cyclostomata, the Japanese river lamprey (Lampetra japonica) has a retina consisting of distinct types of photoreceptor cells called long and short photoreceptor cells. After freeze-fracture, disc membranes of these photoreceptor cells were characterized in common by a homogeneous distribution of intramembrane particles on the protoplasmic fracture faces, in contrast to those of the myeloid bodies bearing scattering particles.Immunofluorescent examination was applied to the retina with monoclonal antibodies raised against bovine and chicken rhodopsins. Positive immunoreactivity was found to be limited to outer segments of the short cell, leaving the entire body of the long cell and all other components of the retina negative. The results suggest that the short cell is more closely related to a rod-type photoreceptor cell characterized by rhodopsin as its visual pigment.
Keywords:Retina  Photoreceptor cells  Freeze-fracturing  Ultrastructure  Immunocytochemistry  Lampetra japonica
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