| 维生素C对视网膜色素上皮蓝光损伤保护作用的研究（简报） |
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| 引用本文： | 周建维 任国良 张晓明 朱晞 林海燕 周吉林. 维生素C对视网膜色素上皮蓝光损伤保护作用的研究（简报）[J]. 实验生物学报, 2003, 36(5): 397-400 |
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| 作者姓名： | 周建维 任国良 张晓明 朱晞 林海燕 周吉林 |
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| 作者单位： | [1]浙江大学医学院附属二院，杭州310009 [2]浙江大学医学院解剖研究室，杭州310031 [3]美国哥伦比亚大学医学院 |
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| 摘 要： | 维生素C为6碳多羟化合物，在化学反应中易失去电子，依次生成半脱氧抗坏血酸和脱氧抗坏血酸。因此，维生素C可作为自由基清除剂，能迅速与超氧阴离子、氢化氧基、过氧化氢、羟自由基反应，生成抗坏血酸自由基。蓝光作为一种短波长，靠近紫外线频段的光，具有能量高的特点，是自然界中导
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| 关 键 词： | 维生素C 视网膜色素上皮 蓝光损伤 DNA |
Study of blue light induced DNA damage of retinal pigment epithelium(RPE) cells and the protection of vitamin C] |
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| Jian Wei Zhou,Guo Liang Ren,Xiao Ming Zhang,Xi Zhu,Hai Yan Lin,Ji Lin Zhou. Study of blue light induced DNA damage of retinal pigment epithelium(RPE) cells and the protection of vitamin C][J]. Acta Biologiae Experimentalis Sinica, 2003, 36(5): 397-400 |
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| Authors: | Jian Wei Zhou Guo Liang Ren Xiao Ming Zhang Xi Zhu Hai Yan Lin Ji Lin Zhou |
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| Affiliation: | Second Affiliated Hospital of Medical School, Zhejiang University, Hangzhou 310009. zj219@columbia.edu |
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| Abstract: | To evaluate protection of vitamin C on blue light-induced DNA damage of human retinal pigment epithelium (RPE) cells. The cultured RPE cells were divided into 3 groups: Control group (no blue light exposure), blue light exposure group (blue light exposure for 20 minutes) and blue light exposure + vitamin C group (blue light exposure + 100 mumol/L vitamin C). Travigen's comet assay kit and Euclid comet assay software were used to assay the DNA damage levels. The DNA percentage in the tail of electrophoretogram in the three groups were 18.44%, 54.42% and 32.43% respectively (p < 0.01). Tail moments were 8.2, 48.3, and 18.4 respectively (p < 0.01). Blue light could induce DNA damage to RPE cells but vitamin C could protect the RPE cells from the blue light-induced DNA damage. |
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